We report the association with reproductive phenotypes of a genetic marker mapped to HELB gene, in tropically adapted cattle. The marker is a single nucleotide polymorphism (SNP) in chromosome 5 that is non-synonymous and has a predicted deleterious effect on the protein. Reproductive phenotypes used in these analyses were from a population of the Cooperative Research Centre for Beef Genetic Technologies. Bulls (n = 1,023) were from a mixed-breed population and represented animals not included in the original genome-wide studies (GWAS) that reported SNP associations on chromosome 5 for scrotal circumference (SC). Cows (n = 1,089) were Tropical Composites and most were from the population used in the original GWAS that described SNP associations on chromosome 5 for puberty and post-partum anoestrus interval (PPAI). Animals were genotyped with Taqman assays designed for 3 SNP. The results indicate that the SNP in HELB is significant for SC and PPAI at P < 10^-7 and for heifer puberty (P = 0.0029). This SNP might aid across breed phenotype prediction for reproductive phenotypes given its significance in these cattle with both Bos taurus and Bos indicus ancestry. Future studies should target this HELB SNP in other populations to confirm the associations we described.